Characterizing an siRNA-Containing Lipid-Nanoparticle Prepared by a Microfluidic Reactor: Small-Angle X-ray Scattering and Cryotransmission Electron Microscopic Studies

• DOI: 10.1021/acs.langmuir.0c01079
• Publication Date: 2020-09-29T04:17:47Z
• Authors: Kenichi Aburai; Kentaro Hatanaka; Shin Takano; Shota Fujii; Kazuo Sakurai
• Keywords: Small-angle X-ray scattering; Radius of gyration; Hydrodynamic radius; Field-Flow Fractionation; Multiangle light scattering; Cationic polymerization; Gyration; Particle (ecology)

A new cationic-lipid/siRNA particle that was designed to deliver siRNA investigated by the combination of small-angle X-ray scattering (SAXS), asymmetric field flow fractionation coupled with multiangle light scattering, and cryotransmission electron microscopy (cryo-TEM). The prepared through two-step mixing using a microfluidic technique. In first step, premixed cationic lipid in an EtOH-rich solution. second solution mixed other lipids, followed solvent exchange water. SAXS showed formation siRNA/cationic pair this consisted major part core final particle. relationship between hydrodynamic radius gyration indicated densely packed PEG-rich shell, confirming well-known core–shell model. cryo-TEM ordering structure enhanced as content increased.