Parametric Study of the Factors Influencing Liposome Physicochemical Characteristics in a Periodic Disturbance Mixer

• DOI: 10.1021/acs.langmuir.1c01005
• Publication Date: 2021-07-07T20:36:32Z
• Authors: Rubén R. López; Ixchel Ocampo; Paula G. Font de ...; Luz-María Sánchez; Anas Alazzam; Thupten Tsering; Karl-F. Bergeron; Sergio Camacho-Léon; Julia V. Burnier; Catherine Mounier; Ion Stiharu; Vahé Nerguizian
• Keywords: Micromixer

Liposomes encapsulate different substances ranging from drugs to genes. Control over the average size and distribution of these nanoparticles is vital for biomedical applications since characteristics determine a high degree where liposomes will accumulate in human body. Micromixers enable continuous flow synthesis liposomes, improving control reproducibility. Recently, Dean dynamics-based micromixers, such as periodic disturbance mixer (PDM), have been shown produce controlled-size scalable reproducible way. However, contrary micromixers based on molecular diffusion or chaotic advection, their production factors influence liposome properties not yet addressed thoroughly. In this work, we present comprehensive parametric study effects conditions changing concentration, lipid type, temperature physicochemical liposomes. Numerical models confocal images are used quantitatively qualitatively evaluate mixing performance under relationship with vesicle properties. The total rate (TFR) and, lesser extent, ratio (FRR) distribution. Effects also observed by factors. Moreover, ζ potential independent studied here. micromixer presented work enables small 24 nm, monodispersed low close polydispersed populations well 41 mg/h.