Effects of Mechanical Properties of Lipid Bilayers on the Entry of Cell-Penetrating Peptides into Single Vesicles

• DOI: 10.1021/acs.langmuir.6b03111
• Publication Date: 2017-02-06T23:12:28Z
• Authors: Md. Zahidul Islam; Sabrina Sharmin; Victor Levadnyy; Sayed Ul Alam Shibly; Masahito Yamazaki
• Keywords: 0303 health sciences; 03 medical and health sciences; Surface Properties; Lipid Bilayers; Cell-Penetrating Peptides; Particle Size; Unilamellar Liposomes; Mechanical Phenomena

The translocation of cell-penetrating peptides (CPPs) through plasma membranes living cells is an important physiological phenomenon in biomembranes. To reveal the mechanism underlying a CPP, transportan 10 (TP10), lipid bilayers, we examined effects mechanical properties bilayers on entry carboxyfluorescein (CF)-labeled TP10 (CF-TP10) into giant unilamellar vesicle (GUV) using single GUV method. First, effect lateral tension CF-TP10 GUVs comprising mixture dioleoylphosphatidylglycerol (DOPG) and dioleoylphosphatidylcholine (DOPC) (2/8). entered lumen before membrane permeation Alexa Fluor 647 hydrazide (AF647) from thus pore formation membrane. fraction rate rupture increased with tension. CF-TP10-induced fractional change area continuously time until rupture, but it more slowly than did concentration A high mole cholesterol inhibited by suppressing external to internal monolayer, although higher concentrations induced pores which rapidly translocated. Suppression can be reasonably explained large line prepore. We discussed role proposed hypothesis that translocates across bilayer transient hydrophilic prepores