Prediction of Self-Association and Solution Behavior of Monoclonal Antibodies Using the QCM-D Metric of Loosely Interacting Layer

• DOI: 10.1021/acs.molpharmaceut.4c00656
• Publication Date: 2024-11-29T15:21:08Z
• Authors: Yusra Rahman; Siddhanth Hejmady; Reza Nejadnik
• Keywords: Quartz Crystal Microbalance; Association (psychology)

Despite the increasing availability and success of monoclonal antibodies (mAb), early identification candidate molecules with desirable developability attributes remains challenging due to self-association poor solution behavior. Measuring these phenomena experimentally using available methods is complicated in mAbs development. Quartz crystal microbalance dissipation monitoring (QCM-D) detects a loosely interacting layer on top irreversibly adsorbed molecules, providing information about interaction. This work aimed explore whether characteristics this can be used as reliable metric. QCM-D experiments showed large frequency shift (Δ