Regulating the Uptake of Viral Nanoparticles in Macrophage and Cancer Cells via a pH Switch

• DOI: 10.1021/acs.molpharmaceut.8b00348
• Publication Date: 2018-05-22T19:23:46Z
• Authors: Hamilton Lee; Candace E. Benjamin; Chance M. Nowak; Lana H. Tuong; Raymond P. Welch; Zhuo Chen; Madushani Dharmar...; Kyle W. Murray; Leonidas Bleris; Sheena D’Arcy; Jeremiah J. Gasse...
• Keywords: Hexanoic acid; Moiety; Conjugate; HeLa

Controlling the uptake of nanomaterials into phagocytes is a challenging problem. We describe an approach to inhibit cellular by macrophages and HeLa cells nanoparticles derived from bacteriophage Qβ conjugating negatively charged terminal hexanoic acid moieties onto its surface. Additionally, we show hydrazone linkers can be installed between surface moieties, resulting in pH-responsive conjugate that, acidic conditions, release moiety allow for nanoparticle. The installation "pH switch" did not change structure–function properties conjugates macrophages.