DNA Nanoswitch Barcodes for Multiplexed Biomarker Profiling

• DOI: 10.1021/acs.nanolett.0c03929
• Publication Date: 2021-01-04T20:14:19Z
• Authors: Arun Richard Chan...; Molly MacIsaac; Javier Vilcapoma; Clinton H. Hansen; Darren Yang; Wesley P. Wong; Ken Halvorsen
• Keywords: Cancer Biomarkers; Barcode; Biomarker Discovery

Molecular biomarkers play a key role in the clinic, aiding diagnostics and prognostics, research laboratory, contributing to our basic understanding of diseases. Detecting multiple diverse molecular within single accessible assay would have great utility, providing more comprehensive picture for clinical evaluation research, but is challenge with standard methods. Here, we report programmable DNA nanoswitches multiplexed detection up 6 at once each combination producing unique barcode signature among 64 possibilities. As defining feature method, show "mixed multiplexing" simultaneous barcoded different types biomolecules, example, DNA, RNA, antibody, protein assay. To demonstrate potential, prostate cancer biomarker panel serum that includes two microRNA sequences specific antigen.