Deciphering Protein Corona by scFv-Based Affinity Chromatography

• DOI: 10.1021/acs.nanolett.0c04806
• Publication Date: 2021-02-22T18:32:46Z
• Authors: Yuxiu Chu; Wenjing Tang; Zui Zhang; Cheng Li; Jun Qian; Xiaoli Wei; Tianlei Ying; Weiyue Lu; Changyou Zhan
• Keywords: Corona (planetary geology); Protein A

It remains challenging to precisely decipher the structural and functional characteristics of protein coronas. To overcome drawbacks frequently occurring in traditional separation methods, an anti-PEG single-chain variable fragment (PEG-scFv) based affinity chromatography (AfC) was developed achieve precise efficient coronas on PEGylated liposomes (sLip). His-tagged PEG-scFv could readily capture sLip without affecting corona compositions, separate sLip/protein complex from plasma aggregates endogenous vesicles through Ni-NTA column. AfC demonstrated 43-fold higher collecting efficiency than centrifugation, which extremely crucial for vivo due limitation sample size. evaded contamination by reserved loosely bound proteins, providing unprecedented approach deeply The scFv-based also paves new avenues formed other nanomedicines.