Selective Encapsulation of Therapeutic mRNA in Engineered Extracellular Vesicles by DNA Aptamer

• DOI: 10.1021/acs.nanolett.1c01817
• Publication Date: 2021-10-15T20:54:37Z
• Authors: Siyan Zhang; Yan Dong; Yixiao Wang; Wenqi Sun; Mengying Wei; Lijun Yuan; Guodong Yang
• Keywords: Aptamer; Exosome

Extracellular vesicles (EV)-based delivery of therapeutic mRNAs is challenged by the low loading efficiency. In this study, we designed a DNA aptamer consisting two parts: single strand part recognized AUG region target mRNA, preventing mRNA from translation and ribosome assembly; double containing elements CD9-ZF (zinc finger) motifs, sorting aptamer-mRNA complex into engineered EVs. vitro in vivo studies revealed that system could efficiently load functional to Furthermore, adipose specific loaded Pgc1α via strategy induce white adipocyte browning. Similarly, interleukin-10 (Il-10) had potent anti-inflammatory effect inflammatory bowel disease (IBD) mouse model. Together, our study has proposed an efficient interest EVs, which be used as promising for gene therapy.