Quantum Sensing of Free Radicals in Primary Human Dendritic Cells

• DOI: 10.1021/acs.nanolett.1c03021
• Publication Date: 2021-12-21T00:38:35Z
• Authors: Linyan Nie; Anggrek C. Nusantara; Viraj G. Damle; Maxim V. Baranov; Mayeul Chipaux; Claudia Reyes-San...; Thamir Hamoh; Chandra Prakash E...; Miroslava Guricova; Petr Cigler; Geert van den Bog...; Romana Schirhagl
• Keywords: Primary cell

Free radicals are crucial indicators for stress and appear in all kinds of pathogenic conditions, including cancer, cardiovascular diseases, infection. However, they difficult to detect due their reactivity low abundance. We use relaxometry the detection with subcellular resolution. This method is based on a fluorescent defect diamond, which changes its optical properties basis magnetic surroundings. technique allows nanoscale MRI unprecedented sensitivity spatial Recently, this was used inside living cells from cell line. Cell lines differ terms endocytic capability radical production primary derived patients. Here we provide first measurements phagocytic by NADPH oxidase (NOX2) dendritic healthy donors. The these differs greatly between investigated response stimulation or inhibition.