High Spatial and Temporal Resolution NIR-IIb Gastrointestinal Imaging in Mice

• DOI: 10.1021/acs.nanolett.1c04909
• Publication Date: 2022-03-25T00:43:41Z
• Authors: Chao Mi; Ming Guan; Xun Zhang; Liu Yang; Sitong Wu; Zhichao Yang; Zhiyong Guo; Jiayan Liao; Jiajia Zhou; Fulin Lin; En Ma; Dayong Jin; Xiaocong Yuan
• Keywords: Temporal resolution; Autofluorescence

Conventional biomedical imaging modalities, including endoscopy, X-rays, and magnetic resonance, are invasive cannot provide sufficient spatial temporal resolutions for regular of gastrointestinal (GI) tract to guide prognosis therapy GI diseases. Here we report a non-invasive method optical tract. It is based on new type lanthanide-doped nanocrystal with near-infrared (NIR) excitation at 980 nm second NIR window (NIR-IIb) (1500~1700 nm) fluorescence emission around 1530 nm. The rational design controlled synthesis nanocrystals high brightness have led an absolute quantum yield (QY) up 48.6%. Further benefitting from the minimized scattering through NIR-IIb window, enhanced resolution by 3 times compared other NIR-IIa (1000~1500 contract agents imaging. approach also 8 frames per second, so that moment mice intestinal peristalsis happened in one minute can be captured. Furthermore, light-sheet system, demonstrated three-dimensional (3D) stereoscopic structure Moreover, successfully translate these advances diagnose inflammatory bowel disease (IBD) pre-clinical model colitis.