Implantable Aptamer-Graphene Microtransistors for Real-Time Monitoring of Neurochemical Release in Vivo

• DOI: 10.1021/acs.nanolett.2c00289
• Publication Date: 2022-04-19T21:48:31Z
• Authors: Guangfu Wu; Nannan Zhang; Avi Matarasso; Ian Heck; Huijie Li; Wei Lu; J. Glenn Phaup; Michael J. Schneider; Yixin Wu; Zhengyan Weng; He Sun; Zan Gao; Xincheng Zhang; Stefan G. Sandberg; Dilruba Parvin; Elena Seaholm; Syed Kamrul Islam; Xueju Wang; Paul E. M. Phillips; Daniel C. Castro; Shinghua Ding; De-Pei Li; Michael R. Bruchas; Yi Zhang
• Keywords: Aptamer; Neurochemical

The real-time monitoring of neurochemical release in vivo plays a critical role understanding the biochemical process complex nervous system. Current technologies for such applications, including microdialysis and fast-scan cyclic voltammetry, suffer from limited spatiotemporal resolution or poor selectivity. Here, we report soft implantable aptamer-graphene microtransistor probe release. As demonstration, show dopamine with nearly cellular-scale spatial resolution, high selectivity (dopamine sensor >19-fold over norepinephrine), picomolar sensitivity, simultaneously. Systematic benchtop evaluations, ex experiments, studies mice models highlight key features demonstrate capability capturing dynamics evoked by pharmacological stimulation, suggesting potential applications basic neuroscience studying neurological disease-related processes. developed system can be easily adapted other neurochemicals drugs simply replacing aptamers functionalized on graphene microtransistors.