Developing a Ready-to-Use Lipid Nanoparticle Technology for Nucleic Acid Delivery Based on Deep Eutectic Solvents

• DOI: 10.1021/acs.nanolett.4c04665
• Publication Date: 2024-11-23T01:47:58Z
• Authors: Yuning Wei; Weiwen Kong; Zirong Dong; Wenjuan Liu; Xinrui Lu; Peiheng Yang; Jiaxin Zhao; Jiayi Feng; Zibo Wei; Jinlong Yang; Jianping Qi
• Keywords: Solid lipid nanoparticle

Microfluidic technology has emerged as a prevalent tool to produce lipid nanoparticles (LNPs) for nucleic acid delivery. However, its wide-ranging application is hindered by specialized, costly equipment and consumables. Herein, ready-to-use nanoparticle (RULNP) employing deep eutectic solvents (DESs) was developed. The DES, consisting of fructose glycerol ([Fru][Gly]), able dissolve lipids acids, facilitating the formation RULNPs simple physical mixing hydrating. This innovative approach circumvents high costs organic associated with microfluidic methods offers flexibility in preparation techniques, accommodating various scenarios. exhibited physicochemical properties plasmid DNA (pDNA) or RNA delivery efficacy comparable those LNPs. Mechanistic studies revealed that achieved superior cellular uptake compared LNPs despite exhibiting limited endosomal escape capabilities. Collectively, DES-based RULNP system presents rapid straightforward method LNP production, potentially revolutionizing