Rational Design of a Polymer with Robust Efficacy for Intracellular Protein and Peptide Delivery

• DOI: 10.1021/acs.nanolett.6b04955
• Publication Date: 2017-02-16T16:42:58Z
• Authors: Hong Chang; Jia Lv; Xin Gao; Xing Wang; Hui Wang; Hui Chen; Xu He; Lei Li; Yiyun Cheng
• Keywords: Rational design; Saporin

The efficient delivery of biopharmaceutical drugs such as proteins and peptides into the cytosol target cells poses substantial challenges owing to their large size susceptibility degradation. Current protein vehicles have limitations need for modification, insufficient large-size or small peptides, loss function after delivery. Here, we adopted a rational approach design polymer with robust efficacy intracellular peptide is composed dendrimer scaffold, hydrophobic membrane-disruptive region, multivalent binding surface. It allows protein/peptide binding, endocytosis, endosomal disruption capable efficiently delivering various biomacromolecules including bovine serum albumin, R-phycoerythrin, p53, saporin, β-galactosidase, living cells. Transduction apoptotic successfully induces apoptosis in cancer cells, suggesting that activities are maintained during This technology represents an useful tool has broad applicability basic research clinical applications.