Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes

• DOI: 10.1021/acs.nanolett.7b01523
• Publication Date: 2017-05-23T09:37:52Z
• Authors: Michele Dipalo; Hayder Amin; Laura Lovato; Fabio Moia; Valeria Caprettini; Gabriele C. Messina; Francesco Tantussi; Luca Berdondini; Francesco De Angelis
• Keywords: SIGNAL (programming language)

Three-dimensional vertical micro- and nanostructures can enhance the signal quality of multielectrode arrays promise to become prime methodology for investigation large networks electrogenic cells. So far, access intracellular environment has been obtained via spontaneous poration, electroporation, or by surface functionalization micro/nanostructures; however, these methods still suffer from some limitations due their intrinsic characteristics that limit widespread use. Here, we demonstrate ability continuously record both extracellular intracellular-like action potentials at each electrode site in spontaneously active mammalian neurons HL-1 cardiac-derived cells combination nanoelectrodes with plasmonic optoporation. We long-term stable recordings a very good signal-to-noise ratio. Additionally, optoporation does not perturb electrical activity; it permits continuous recording even during poration process regulate contributions means partial cellular poration.