Dually Gated Polymersomes for Gene Delivery

• DOI: 10.1021/acs.nanolett.8b01985
• Publication Date: 2018-07-27T17:25:47Z
• Authors: Fangyingkai Wang; Jingyi Gao; Jiangang Xiao; Jianzhong Du
• Keywords: Polymersome

An ideal gene carrier requires an excellent gating system to efficiently load, protect, deliver, and release environmentally sensitive nucleic acids on demand. Presented in this communication is a polymersome with "boarding gate" "debarkation the membrane complete above important missions. This dually gated self-assembled from block copolymer, poly(ethylene oxide)-block-poly[N-isopropylacrylamide-stat-7-(2-methacryloyloxyethoxy)-4-methylcoumarin-stat-2-(diethylamino)ethyl methacrylate] [PEO-b-P(NIPAM-stat-CMA-stat-DEA)]. The hydrophilic PEO chains form coronas of polymersome, whereas temperature pH-sensitive P(NIPAM-stat-CMA-stat-DEA) forms heterogeneous membrane. temperature-controlled can be opened at room for facile encapsulation siRNA plasmid DNA into polymersomes directly aqueous solution. triggered by proton sponge effect intracellular release. Biological studies confirmed successful DNA, efficient vitro vivo transfection, expression green fluorescent protein (GFP) GFP-encoding plasmid, suggesting that kind dual serve as biomacromolecular shuttle delivery other biological applications.