Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate

• DOI: 10.1021/acs.nanolett.8b03342
• Publication Date: 2018-09-25T14:28:22Z
• Authors: Weixiang Ye; Markus Götz; Sirin Celiksoy; Laura Tüting; Christoph Ratzke; Janak Prasad; Julia Ricken; Seraphine V. Wegner; Rubén Ahijado-Guzmán; Thorsten Hugel; Carsten Sönnichsen
• Keywords: Dynamics

We use plasmon rulers to follow the conformational dynamics of a single protein for up 24 h at video rate. The ruler consists two gold nanospheres connected by linker. In our experiment, we molecular chaperone heat shock 90 (Hsp90), which is known show "open" and "closed" conformations. Our measurements confirm previously with transition times in second minute time scale reveals new on minutes hours. Plasmon thus extend observation bandwidth 3–4 orders magnitude respect single-molecule fluorescence resonance energy transfer enable study unprecedented precision.