A Cryptic Palmitoyl Chain Involved in Safracin Biosynthesis Facilitates Post-NRPS Modifications

• DOI: 10.1021/acs.orglett.1c03741
• Publication Date: 2021-12-09T19:33:22Z
• Authors: Ying-Ying Zhang; Ning Shao; Wan-Hong Wen; Gong-Li Tang
• Keywords: 0301 basic medicine; 03 medical and health sciences; Peptide Synthases

This study confirmed the participation of a cryptic palmitoyl fatty acyl chain in the biosynthesis of safracin and unraveled a previously ignored peptidase for the removal of the precursor. Furthermore, the post-assembly line tailoring steps are extensively studied in terms of the methyltransferase SacI-catalyzed N-methylation and the FAD-dependent monooxygenase SacJ-catalyzed A-ring oxidation. The timing of these post-NRPS steps is also addressed in this work.