Discovery and Biosynthesis of Atrovimycin, an Antitubercular and Antifungal Cyclodepsipeptide Featuring Vicinal-dihydroxylated Cinnamic Acyl Chain

• DOI: 10.1021/acs.orglett.9b00618
• Publication Date: 2019-04-08T14:43:28Z
• Authors: Qing Liu; Zhiyong Liu; Changli Sun; Mingwei Shao; Junying Ma; Xiaoyi Wei; Tianyu Zhang; Wenjun Li; Jianhua Ju
• Keywords: Hydroxylation; Moiety; Vicinal

Atrovimycin (1), a cyclodepsipeptide containing unique vicinal-hydroxylated cinnamic acyl chain, was isolated and elucidated from Streptomyces atrovirens LQ13. The biosynthetic pathway of 1 achieved, revealing cytochrome P450 (Avm43) epoxide hydrolase (Avm29) enzymes constructing the vicinal-dihydroxy substitution, as well tailoring (Avm28) enzyme catalyzing β-hydroxylation l-Phe moiety. shows in vitro antifungal activity antitubercular against Mycobacterium tuberculosis H37Rv both (with MIC 2.5 μg/mL) vivo.