Electric Field Polarity Controls Distribution of Viral Bioreceptors within Near-Field Electrospun Biohybrid Microfiber Optical Biosensors

• DOI: 10.1021/acsabm.4c01761
• Publication Date: 2025-02-15T16:49:01Z
• Authors: Stephen T. Hsieh; Jordyn M. Watkins; Zaira Alibay; Joshua M. Plank; Kalie Inouye; Nosang V. Myung; Elaine D. Haberer
• Keywords: Microfiber; Polarity (international relations)

Microorganisms (e.g., bacteria, fungi, and viruses) add indispensable functionality to a range of electrospun polymer materials devices. The optimal distribution bioactive agents on either the interior or exterior fiber is application-specific. Current microbe surface immobilization strategies core-confinement techniques continue pose number challenges. Here, we explore simple strategy, utilizing electrostatic forces, control migration concentration M13 bacteriophage within near-field poly(vinyl alcohol) (PVA) microfibers. Both charge virus applied electric field polarity altered placement. When doped with Rhodamine 6G (R6G), circular microfiber cross sections formed active whispering gallery mode (WGM) resonators. These relatively high-quality (Q) optical cavities enabled us sensitively probe content their outer layer, while functioning as label-free biosensors phage-based streptavidin biorecognition elements. Coulomb forces displayed significant over coverage during electrospinning, increasing biosensor response by nearly factor 4 1310 nM streptavidin. findings are an important demonstration simple, yet adaptable method enhance biohybrid performance tailoring distribution.