Cold to Hot: Binary Cooperative Microneedle Array-Amplified Photoimmunotherapy for Eliciting Antitumor Immunity and the Abscopal Effect

• DOI: 10.1021/acsami.0c05090
• Publication Date: 2020-05-14T11:48:01Z
• Authors: Minglong Chen; Guilan Quan; Ting Wen; Peipei Yang; Wanbing Qin; Haoting Mai; Ying Sun; Chao Lu; Xin Pan; Chuanbin Wu
• Keywords: Indocyanine Green; Immune checkpoint

In this study, an ingenious core–shell structure microneedle (CSMN) array was designed to synergistically boost robust immune response by the intralesional codelivery of photosensitizer and indoleamine 2,3-dioxygenase (IDO) blockade. Photosensitizer indocyanine green encapsulated into chitosan nanoparticles (ICG-NPs), followed concentrating on tip shell microneedles. 1-Methyl-tryptophan loaded cross-linked poly(vinyl pyrrolidone) alcohol) gel as core. Through direct deposition ICG-NP-loaded tips tumor site with uniform spatial distribution, CSMNs effectively converted near-infrared laser heat ablate primary tumors, generated tumor-associated antigens damage-associated molecular patterns, promoted maturation dendritic cells secretion immunostimulatory cytokines. The IDO blockade further reversed IDO-mediated immunosuppression, ultimately arousing effective systematic response. in vivo results showed that 80% melanoma eradicated, a relapse-free survival more than 120 days. Of note, synergistic strategy significantly inhibited lung metastasis controlled development already metastasized tumors. Our work provides new, generalizable framework for using microneedle-based photothermal therapy initiate antitumor immunity sensitize tumors