Cuboplex-Mediated Nonviral Delivery of Functional siRNA to Chinese Hamster Ovary (CHO) Cells

• DOI: 10.1021/acsami.0c20956
• Publication Date: 2021-01-10T05:44:36Z
• Authors: Sampa Sarkar; Nhiem Tran; Sarvesh Kumar Soni; Zeyad Nasa; Calum J. Drummond; Charlotte E. Conn
• Keywords: Drug Carriers; Green Fluorescent Proteins; CHO Cells; Transfection; Lipids; 01 natural sciences; Glycerides; 0104 chemical sciences; 3. Good health; Cricetulus; Cations; Animals; RNA Interference; RNA, Small Interfering

Lipid nanoparticles of internal cubic symmetry, termed cuboplexes, are potential nonviral delivery vehicles for gene therapy due to their "topologically active" nature, which may enhance endosomal escape and improve outcomes. In this study, we have used cationic based on monoolein (MO) doped with a lipid, the encapsulation antisense green fluorescent protein (GFP)—small interfering RNA (siRNA) into Chinese Hamster Ovary (CHO)—GFP cells. Agarose gel electrophoresis has confirmed successful siRNA within cubosomes, while synchrotron small-angle X-ray scattering (SAXS) demonstrated that underlying nanostructure particles was retained following encapsulation. The cubosomes were shown be reasonably nontoxic against CHO-GFP cell line. Fluorescence-activated sorting (FACS) provided evidence transfection Knockdown efficiency strongly linked type lipid used, although all had essentially same nanostructure. knockdown some higher than lipofectamine, is commercially available liposome-based formulation, controlled release from over 72 h period observed using confocal microscopy. This combination exemplifies cuboplexes as novel, nonviral, controlled-release vector siRNA.