mRNA-Activated Multifunctional DNAzyme Nanotweezer for Intracellular mRNA Sensing and Gene Therapy

• DOI: 10.1021/acsami.0c21601
• Publication Date: 2021-02-10T10:34:44Z
• Authors: Manman He; Mengyun He; Cunpeng Nie; Jintao Yi; Juan Zhang; Tingting Chen; Xia Chu
• Keywords: Deoxyribozyme; Survivin

Deoxyribozyme (DNAzyme) is regarded as a promising gene therapy drug. However, poor cellular uptake efficacy and low biological stability limit the utilization of DNAzyme in therapy. Here, we report well-known programmable DNAzyme-based nanotweezer (DZNT) that provides new strategy for detection TK1 mRNA survivin mRNA-targeted silencing At end DZNT arm, there are two functionalized single-stranded DNA each consists parts: segment complementary to split-DNAzyme segment. The hybridization with intracellular enables imaging mRNA. Meanwhile, draws close other activates cleave realize results demonstrate nanocarrier has excellent cell penetration, good biocompatibility, noncytotoxicity. can image biomolecule high contrast. Furthermore, efficiently aid commonly present cancer cells, accordingly selectively kill no harm normal cells. Taken together, multifunctional platform early diagnosis tumors