Self-Assembling Nano-Globular Peptide from Human Lactoferrin Acts as a Systemic Enhancer of Bone Regeneration: A Novel Peptide for Orthopedic Application

• DOI: 10.1021/acsami.1c01513
• Publication Date: 2021-04-15T00:59:02Z
• Authors: Subhashis Pal; Mohd Sayeed; Amit Kumar; Devesh P. Verma; Munesh K. Harioudh; Neeraj K. Verma; Konica Porwal; Shivani Sharma; Chirag Kulkarni; Amitabha Bandyopa...; Madhav N. Mugale; Kalyan Mitra; Jimut K. Ghosh; Naibedya Chattopa...
• Keywords: Lactoferrin; Teriparatide

A technology for systemic and repeated administration of osteogenic factors orthopedic use is an unmet medical need. Lactoferrin (∼80 kDa), present in milk, known to support bone growth. We discovered a lactoferrin-mimetic peptide, LP2 (an 18-residue fragment from the N-terminus N-lobe human lactoferrin), which self-assembles into nano-globular assembly with β-sheet structure aqueous environment. non-hemolytic non-cytotoxic against red blood cells 3T3 fibroblasts, respectively, appreciably stable serum. through morphogenetic protein-dependent mechanism stimulates osteoblast differentiation more potently than full-length protein as well osteoblastic production osteoprotegerin anti-osteoclastogenic factor). Consequently, daily subcutaneous rats rabbits osteotomy resulted faster healing stimulated formation low mass that teriparatide, standard-of-care peptide osteoporosis. has skeletal bioavailability safe at 15× dose. Thus, novel can be administered systemically management hard-to-heal fractures.