Biocompatible and Photostable Photoacoustic Contrast Agents as Nanoparticles Based on Bodipy Scaffold and Polylactide Polymers: Synthesis, Formulation, and In Vivo Evaluation

• DOI: 10.1021/acsami.2c04874
• Publication Date: 2022-08-31T17:26:01Z
• Authors: Jean-Baptiste Bodin; Jérôme Gateau; Justine Coïs; Théotim Lucas; Flora Lefebvre; Laurence Moine; Magali Noiray; Catherine Cailleau; Stéphanie Denis; Gilles Clavier; Nicolas Tsapis; Rachel Méallet-Re...
• Keywords: BODIPY; Cyanine

We have designed a new Bodipy scaffold for efficient in vivo photoacoustic (PA) imaging of nanoparticles commonly used as drug nanovectors. The dye has an optimized absorption band the near-infrared window biological tissue and low fluorescence quantum yield that leads to good generation efficiency. After Bodipy-initiated ring-opening polymerization lactide, polylactide-Bodipy was formulated into PEGylated (NPs) by mixing with PLA-PEG at different concentrations. Formulated NPs around 100 nm exhibit excellent PA properties: 760 molar coefficient between molecular absorbers gold NPs. Highly improved photostability compared cyanine-labeled PLA well innocuity cultured macrophages were demonstrated. intravenous injection healthy animals, easily detected using commercial system spectral unmixing, opening way their use theranostic agents.