Stepwise Multi-Cross-Linking Bioink for 3D Embedded Bioprinting to Promote Full-Thickness Wound Healing

• DOI: 10.1021/acsami.3c00688
• Publication Date: 2023-05-10T20:20:34Z
• Authors: Lili Hao; Xiwang Tao; Miao Feng; Ke Zhou; Yiyan He; Jun Yang; Hongli Mao; Zhongwei Gu
• Keywords: 3D bioprinting

The emergence and innovation of three-dimensional (3D) bioprinting provide new development opportunities for tissue engineering regenerative medicine. However, how to obtain bioinks with both biomimicry manufacturability remains a great issue in 3D bioprinting. Developing intelligent responsive biomaterials is conducive break through the current dilemma. Herein, stepwise multi-cross-linking strategy concerning thermosensitive thiolated Pluronic F127 (PF127-SH) hyaluronic acid methacrylate (HAMA) proposed achieve temperature-controlled embedded bioprinting, specifically pre-cross-linking (Michael addition reaction) at low temperatures (4-20 °C) subsequently self-assembly (hydrophobic interaction) high-temperature (30-37 suspension bath as well final photo-cross-linking (mainly thiol-ene "click" reaction). unique cross-linking mechanism promises bioink appropriate viscosity different printing stages, making it possible print complex structures excellent shape fidelity simultaneously maintain biological activity cells. In vitro studies reveal that 3D-printed hydrogels are beneficial enhancing cell viability. Further, vivo experiments demonstrate cell-laden printed significantly promote wound healing re-epithelialization by modulating inflammation accelerating collagen deposition angiogenesis. Therefore, expected accelerate novel clinical applications