Harnessing Bioluminescent Bacteria to Develop an Enzymatic-free Enzyme-linked immunosorbent assay for the Detection of Clinically Relevant Biomarkers

• DOI: 10.1021/acsami.4c01744
• Publication Date: 2024-04-23T13:12:50Z
• Authors: Liming Hu; Marianna Rossetti; José Francisco Be...; Claudio Parolo; Ruslan Álvarez-Diduk; Lourdes Rivas; Andrea Idili; Arben Merkoçi
• Keywords: Inner filter effect; Point-of-care diagnostics; Homogeneous immunoassay; Human IgG; Settore CHIM/01; inner filter effect; human IgG; Nanoparticles; nanoparticles; point-of-care diagnostics; SARS-CoV-2 nucleoprotein; nanomaterials; homogeneous immunoassay; Nanomaterials

Enzyme-linked immunosorbent assay (ELISA) is the gold standard technique for measuring protein biomarkers due to its high sensitivity, specificity, and throughput. Despite success, continuous advancements in ELISA immunoassay formats are crucial meet evolving global challenges address new analytical needs diverse applications. To expand capabilities applications of immunoassays, we introduce a novel ELISA-like that call Bioluminescent-bacteria-linked (BBLISA). BBLISA an enzyme-free utilizes inner filter effect between bioluminescent bacteriaAllivibrio fischeriand metallic nanoparticles (gold iridium oxide nanoflowers) as molecular absorbers. Functionalizing these with antibodies induces their accumulation wells upon binding targets, forming classical immune-sandwich complex. Thanks ability adsorb light emitted by bacteria, can suppress bioluminescence signal, allowing rapid quantification target. demonstrate bioanalytical properties platform, proof principle, detected two clinically relevant (human immunoglobulin G SARS-CoV-2 nucleoprotein) human serum, achieving same sensitivity precision classic ELISA. We believe be promising alternative techniques, offering potential biomarker detection analysis combining nanomaterials low-cost, portable platform.