Studying Different Binding and Intracellular Delivery Efficiency of ssDNA Single-Walled Carbon Nanotubes and Their Effects on LC3-Related Autophagy in Renal Mesangial Cells via miRNA-382

• DOI: 10.1021/acsami.5b07185
• Publication Date: 2015-09-01T16:46:14Z
• Authors: Guobao Wang; Tingting Zhao; Leyu Wang; Bianxiang Hu; Ali Darabi; Jiansheng Lin; Malcolm M. Q. Xing; Xiaozhong Qiu
• Keywords: 0303 health sciences; Base Sequence; Nanotubes, Carbon; Molecular Sequence Data; Gene Transfer Techniques; Intracellular Space; DNA, Single-Stranded; Fluorescent Antibody Technique; Kidney; Coculture Techniques; Mice; MicroRNAs; 03 medical and health sciences; Cell Line, Tumor; Mesangial Cells; Autophagy; Animals; Humans; Nucleic Acid Conformation; Thermodynamics; Microtubule-Associated Proteins

Single-walled carbon nanotubes (SWCNTs) have been used to deliver single-stranded (ssDNA). ssDNA in oligonucleotide can act as an inhibitor of microRNA regulate cellular functions. However, these are difficult bind with low transferring efficiency cells. To this end, we designed regulatory and functional units form ssDNA-SWCNT hybrids study their binding effects efficiency. The unit on mimics the (MI) miRNA-382, which plays a crucial role progress many diseases such renal interstitial fibrosis. After verification overexpression miRNA-382 coculture system, sequences (GCG)5-MI, (TAT)5-MI, N23-MI added 5′-terminal end DNA fragment, respectively. These lead different secondary structures thus exhibit affinity ability SWCNTs, finally decide efficacy Autophagy, apoptosis necrosis were observed mesangial