Biogenic Nanoselenium Particles Effectively Attenuate Oxidative Stress-Induced Intestinal Epithelial Barrier Injury by Activating the Nrf2 Antioxidant Pathway

• DOI: 10.1021/acsami.7b03377
• Publication Date: 2017-04-13T09:13:09Z
• Authors: Deguang Song; Yuanzhi Cheng; Xiaoxiao Li; Fengqin Wang; Zeqing Lu; Xiao Xiao; Yizhen Wang
• Keywords: Intestines; Mice; Oxidative Stress; Selenium; 0303 health sciences; 03 medical and health sciences; NF-E2-Related Factor 2; Swine; Animals; Antioxidants

In the present study, a new form of selenium nanoparticle (biogenic nanoselenium (BNS) particles) was synthesized using bacteria. The protection BNS particles against oxidative stress-induced intestinal barrier dysfunction and inherent mechanisms this process were investigated, selenomethionine (SeMet) chemically (Nano-Se) used for comparison. Characterization revealed that they monodispersed homogeneous spheres, with an average size 139.43 ± 7.44 nm. mouse model stress, found to protect function preserve redox homeostasis more efficiently than SeMet Nano-Se. vitro experiments porcine jejunum epithelial (IPEC-J2) cells verified stronger barrier-protecting effect reduced cell apoptosis improved state. activated nuclear factor (erythroid-derived-2)-like 2 (Nrf2) increased expression its downstream genes, including thioredoxin reductase (TXNRD)-1, NADPH dehydrogenase (NQO)-1, heme oxygenase (HO)-1, (Trx), in dose- time-dependent manners. contrast, Nano-Se merely enhanced activity selenoenzymes TXNRD-1 glutathione peroxidase (GPx)-1, indicating role donors. Moreover, knock down Nrf2 significantly blocked antioxidative BNS, confirming protects from damage by activating genes. Our results suggest is promising species potential application treating stress-related diseases.