Bactericidal Effects of Silver Nanoparticles on Lactobacilli and the Underlying Mechanism

• DOI: 10.1021/acsami.7b17274
• Publication Date: 2018-02-26T21:20:56Z
• Authors: Xin Tian; Xiumei Jiang; Cara Welch; Timothy R. Croley; Tit-Yee Wong; Chao Chen; Sanhong Fan; Yu Chong; Ruibin Li; Cuicui Ge; Chunying Chen; Jun-Jie Yin
• Keywords: Lactobacillus; Staphylococcus aureus; Silver; Escherichia coli; Metal Nanoparticles; 02 engineering and technology; 0210 nano-technology; Anti-Bacterial Agents; 3. Good health

While the antibacterial properties of silver nanoparticles (AgNPs) have been demonstrated across a spectrum of bacterial pathogens, the effects of AgNPs on the beneficial bacteria are less clear. To address this issue, we compared the antibacterial activity of AgNPs against two beneficial lactobacilli ( Lactobacillus delbrueckii subsp. bulgaricus and Lactobacillus casei) and two common opportunistic pathogens ( Escherichia coli and Staphylococcus aureus). Our results demonstrate that those lactobacilli are highly susceptible to AgNPs, while the opportunistic pathogens are not. Acidic environment caused by the lactobacilli is associated with the bactericidal effects of AgNPs. Our mechanistic study suggests that the acidic growth environment of lactobacilli promotes AgNP dissolution and hydroxyl radical (•OH) overproduction. Furthermore, increases in silver ions (Ag+) and •OH deplete the glutathione pool inside the cell, which is associated with the increase in cellular reactive oxygen species (ROS). High levels of ROS may further induce DNA damage and lead to cell death. When E. coli and S. aureus are placed in a similar acidic environment, they also become more susceptible to AgNPs. This study provides a mechanistic description of a pH-Ag+-•OH bactericidal pathway and will contribute to the responsible development of products containing AgNPs.