Simultaneous Detection of Antibiotic Resistance Genes on Paper-Based Chip Using [Ru(phen)2dppz]2+ Turn-on Fluorescence Probe

• DOI: 10.1021/acsami.7b17653
• Publication Date: 2018-01-11T16:29:42Z
• Authors: Bofan Li; Xiaoming Zhou; Hongxing Liu; Huaping Deng; Ru Huang; Da Xing
• Keywords: Amplicon

Antibiotic resistance, the ability of some bacteria to resist antibiotic drugs, has been a major global health burden due extensive use agents. resistance is encoded via particular genes; hence specific detection these genes necessary for diagnosis and treatment resistant cases. Conventional methods monitoring require sample be transported central laboratory tedious sophisticated tests, which grueling time-consuming. We developed paper-based chip, integrated with loop-mediated isothermal amplification (LAMP) "light switch" molecule [Ru(phen)2dppz]2+, conduct turn-on fluorescent genes. In this assay, reagents can embedded into test spots chip in advance, thus simplifying procedure. [Ru(phen)2dppz]2+ was applied intercalate amplicons product analysis, enabling assay operated wash-free format. The device exhibited limit (LOD) as few 100 copies Meanwhile, it could detect from various bacteria. Noticeably, approach other besides by simply changing LAMP primers. Therefore, potential point-of-care (POC) applications gene samples, especially resource-limited conditions.