Establishment of a Human iPSC- and Nanofiber-Based Microphysiological Blood–Brain Barrier System

• DOI: 10.1021/acsami.8b03962
• Publication Date: 2018-06-13T14:36:21Z
• Authors: Dianjun Qi; Shaohua Wu; Haishuang Lin; Mitchell A. Kuss; Yuguo Lei; Alexey Krasnoslob...; Shaheen Ahmed; Chi Zhang; Hyung Joon Kim; Peng Jiang; Bin Duan
• Keywords: PLGA; Occludin; Human brain

The blood–brain barrier (BBB) is an active and complex diffusion that separates the circulating blood from brain extracellular fluid, regulates nutrient transportation, provides protection against various toxic compounds pathogens. Creating in vitro microphysiological BBB system, particularly with relevant human cell types, will significantly facilitate research of neuropharmaceutical drug delivery, screening, transport, as well improve our understanding pathologies are due to damage. Currently, most models generated by culturing rodent astrocytes endothelial cells, using commercially available transwell membranes. Those membranes made plastic biopolymers nonbiodegradable, porous, stiff. In addition, distinct astrocytes, possess unique complexity physiology, which among few characteristics differentiate brains brains. this study, we established a novel microphysiologocal consisting three-dimensionally printed holder electrospun poly(lactic-co-glycolic) acid (PLGA) nanofibrous mesh, bilayer coculture derived induced pluripotent stem cells (hiPSCs), on PLGA mesh. This model achieved significant integrity tight junction protein expression, effective permeability sodium fluorescein, higher transendothelial electrical resistance (TEER) comparing mesh-based counterparts. Moreover, hiPSC-derived endothielial promoted expression TEER value. We further verified functions antibrain tumor drugs (paclitaxel bortezomib) neurotoxic peptide (amyloid β 1-42). system study potentially provide new, powerful tool for physiology pathology.