Enhanced Photogeneration of Reactive Oxygen Species and Targeted Photothermal Therapy of C6 Glioma Brain Cancer Cells by Folate-Conjugated Gold–Photoactive Polymer Nanoparticles

• DOI: 10.1021/acsami.8b05252
• Publication Date: 2018-05-22T16:04:07Z
• Authors: Jaber Keyvan Rad; Ali Reza Mahdavian; Samideh Khoei; Sakine Shirvalilou
• Keywords: Zeta potential

Tumor-selective photodynamic therapy is a successful method for ablation of malignant and cancerous cells. Herein, we introduce the design preparation functionalized acrylic copolymer nanoparticles with spiropyran (SP) imidazole groups through facile semicontinuous emulsion polymerization. Then, Au3+ ions were immobilized reduced on their surface to obtain photoresponsive gold-decorated polymer (PGPNPs). The prepared PGPNPs surface-modified folic acid as site-specific tumor cell targeting agent improve intracellular uptake via endocytosis. Fourier transform infrared spectroscopy energy dispersive X-ray analyses, UV–vis spectroscopy, scanning electron microscopy, high-resolution transmission microscopy images employed characterize spectral morphological properties. Fluorescence inductively coupled plasma analysis demonstrated line labeling capability improved efficiency folate-conjugated (FA-PGPNPs) toward rat brain cancer cells (C6 glioma) 71.8% in comparison 28.8% nonconjugated ones. Nonpolar SP are converted zwitterionic merocyanine isomers under UV irradiation at 365 nm conjugation Au exhibited enhanced photogeneration reactive oxygen species (ROS). These confirmed by ROS cytotoxicity evaluation C6 glioma Owing strong plasmon resonance absorption gold nanoparticles, FA-PGPNPs provided elevated local photothermal near-IR 808 nm. multifunctional comprehensive integration prospective materials introduced promising nanoprobes ability, therapy, tracking, therapy.