Affinity-Modulated Molecular Beacons on MoS2 Nanosheets for MicroRNA Detection

• DOI: 10.1021/acsami.8b14035
• Publication Date: 2018-10-02T14:32:52Z
• Authors: Mingshu Xiao; Arun Richard Chan...; Wei Ji; Fan Li; Tiantian Man; Changfeng Zhu; Xizhong Shen; Hao Pei; Qian Li; Li Li
• Keywords: Molecular beacon; Molybdenum disulfide; Nuclease

DNA-functionalized layered two-dimensional transition-metal dichalcogenides have attracted tremendous interest for constructing biosensors in recent years. In this work, we report diblock molecular beacons with poly-cytosine (polyC) tails anchored on molybdenum disulfide (MoS2) nanosheets as probes microRNA detection. The polyC block is adsorbed MoS2 and the beacon available hybridization to target; duplex-specific nuclease provides signal amplification by target recycling. By changing length of polyC, regulate density inhibit adsorption enzyme-cleaved oligonucleotides, thereby leading higher quenching efficiency. PolyC-mediated very low background signal, ultrahigh sensitivity (limit detection ∼3.4 fM), specificity detect a single nucleotide mismatch, selectivity from serum samples. This platform holds great potential quantitative analysis miRNAs clinical diagnosis biomedical research.