Patterning Nanoparticles with DNA Molds

• DOI: 10.1021/acsami.8b22691
• Publication Date: 2019-02-22T12:15:51Z
• Authors: Longfei Liu; Mengxi Zheng; Zhe Li; Qian Li; Chengde Mao
• Keywords: 0303 health sciences; 03 medical and health sciences; Ovalbumin; Surface Properties; Metal Nanoparticles; Nucleic Acid Hybridization; Polylysine; DNA; Gold; Microscopy, Atomic Force; Silicon Dioxide; Oligonucleotide Array Sequence Analysis

We report a nanopatterning strategy in which self-assembled DNA nanostructures serve as structural templates. In previous work, ordering of NPs primarily relied on specific recognition, e.g., DNA-DNA hybridization. Only a few cases have been reported on nonspecific adsorption. Unfortunately, these studies were limited by the integrity and homogeneity of templates and the variety of patterned nanoparticles (NPs). Herein, we have developed a general method to pattern various NPs. The NPs adsorb onto substrate via NP-substrate direct interactions and the substrates are patterned into large arrays (>4 × 4 μm) of tiny, accessible cavities by self-assembled DNA arrays. As a demonstration, DNA templates include tetragonal and hexagonal arrays and the NPs include individual DNA nanomotifs, gold nanoparticles (AuNPs), and proteins. All nanostructures have been confirmed by atomic force microscopy and corresponding fast Fourier transform (FFT) analysis.