High-Efficiency Isolation and Rapid Identification of Heterogeneous Circulating Tumor Cells (CTCs) Using Dual-Antibody-Modified Fluorescent-Magnetic Nanoparticles

• DOI: 10.1021/acsami.9b14051
• Publication Date: 2019-10-02T15:13:27Z
• Authors: Zhili Wang; Na Sun; Hui Liu; Changchong Chen; Pi Ding; Xinmin Yue; Hanqing Zou; Chungen Xing; Renjun Pei
• Keywords: Circulating tumor cell; Isolation

Extreme rarity and inherent heterogeneity of circulating tumor cells (CTCs) result in a tremendous challenge for the CTC isolation from patient blood samples with high efficiency purity. Current approaches mainly rely on epithelial cell adhesion molecule (EpCAM), which may significantly reduce ability to capture CTCs when expression EpCAM is lost or down-regulated epithelial-mesenchymal transition. Here, rapid highly efficient method developed isolate identify heterogeneous using fluorescent-magnetic nanoparticles (F-MNPs). A dual-antibody interface targeting N-cadherin fabricated onto F-MNPs as well mesenchymal whole samples. The poly(carboxybetaine methacrylate) brushes excellent antifouling properties are employed decrease nonspecific adhesion. Moreover, provide prompt identification strategy (F-MNPs+, Hoechst 33342+, CD45-) that can directly gentle one-step processing within 1 h after This has been demonstrated through artificial details.