Parkinson’s Disease Associated α-Synuclein Familial Mutants Promote Dopaminergic Neuronal Death in Drosophila melanogaster

• DOI: 10.1021/acschemneuro.8b00107
• Publication Date: 2018-06-15T19:13:06Z
• Authors: Ganesh M. Mohite; Saumya Dwivedi; Subhadeep Das; Rakesh Kumar; Sravya Paluri; Surabhi Mehra; Neha Ruhela; Arunima S; Narendra Nath Jha; Samir K. Maji
• Keywords: Animals, Genetically Modified; 0303 health sciences; 03 medical and health sciences; Drosophila melanogaster; Cell Death; Dopaminergic Neurons; Mutation; alpha-Synuclein; Animals; Brain; Humans; Parkinson Disease

α-Synuclein (α-Syn) aggregation and amyloid formation are associated with loss of dopaminergic neurons in Parkinson's disease (PD). In addition, familial mutations α-Syn shown to be one the definite causes PD. Here we have extensively studied PD G51D, H50Q, E46K using Drosophila model system. Our data showed that flies expressing mutants a shorter lifespan exhibit more climbing defects compared wild-type (WT) an age-dependent manner. The immunofluorescence studies brain from old neuronal cell death all WT. This adverse effect is highly correlated sustained population oligomer production retention mutant flies. Furthermore, this was supported by our vitro studies, where significantly higher amount observed suggest could major cause mutants.