Screening Antibiotic-Resistant Escherichia coli in Wastewater and River Water Using a Novel Simple Phenotypic Antibiotic-Susceptibility Testing Method

• DOI: 10.1021/acsestwater.1c00359
• Publication Date: 2022-06-27T07:38:38Z
• Authors: Hisashi Satoh; Natsumi Nagahashi; Kai Kikuchi; Reiko Hirano
• Keywords: culture-based method; lethal concentration; β-d-glucuronidase; fate of antibiotic-resistant Escherichia coli; 518; 0207 environmental engineering; microplate reader; 02 engineering and technology; logarithmic (log) growth phase

Recently, wastewater treatment plants have been identified as potential reservoirs of antibiotic-resistant bacteria. Hence, it is important to monitor bacteria in plants. Currently available methods are laborious, time-consuming, and costly. In this study, a novel simple phenotypic antibiotic-susceptibility testing method for Escherichia coli was developed. A growth curve E. the presence an antibiotic established by monitoring fluorescence intensity fluorogenic substrate specific using microplate reader. The total ratios river water samples were determined measurements. lethal concentrations antibiotics could be roughly estimated method. dependent on type rather than differences between higher from primary clarifiers secondary decreased distance plant discharge outlet increased. allows rapidly with high throughput will allow effective timely decisions made control coli.