Analysis of Unamplified 16S rRNA of Ammonia-Oxidizing Bacteria in Activated Sludge by Spectrophotometry Using Gold Nanoprobes

• DOI: 10.1021/acsestwater.3c00265
• Publication Date: 2023-08-23T15:09:45Z
• Authors: Meri Nakajima; Reiko Hirano; Yuki Nakaya; Hisashi Satoh

The identification of the bacteria contributing to water purification processes in these systems is crucial for development and optimization wastewater treatment by engineers. We developed a simple assay colorimetric quantification bacterial 16S rRNA functional extracted from samples without reverse transcription RNA or quantitative polymerase chain reaction (qPCR). heat test sample significantly reduced time (<3 min) improved sensitivity. Following optimization, calibration curve ammonia-oxidizing (AOB) β subdivision class Proteobacteria was created using activated sludge samples. linear above 1.9 × 106 copies/mL AOB, which 2 orders magnitude lower than that our previous studies. Finally, we determined abundance AOB compared results obtained transcription-qPCR. There some correlation concentrations both methods. Thus, have successfully an innovative tool quantify concentration processes.