Immunogenicity Assessment of Cell Wall Carbohydrates of Group A Streptococcus via Self-Adjuvanted Glyco-lipopeptides

• DOI: 10.1021/acsinfecdis.0c00722
• Publication Date: 2021-02-06T11:59:51Z
• Authors: Farjana Khatun; Charles C. Dai; Tania Rivera-Hern...; Waleed M. Hussein; Zeinab G. Khalil; Robert J. Capon; Istvan Toth; Rachel J. Stephenson
• Keywords: Lipopeptide; Antigenicity; Rhamnose

Identifying the immunogenic moieties and their precise structure of carbohydrates plays an important role for developing effective carbohydrate-based subunit vaccines. This study assessed structure-immunogenicity relationship carbohydrate a single repeating unit group A (GAC) present on cell wall Streptococcus (GAS) using rationally designed self-adjuvanted lipid-core peptide, instead carrier protein. Immunological evaluation fully synthetic glyco-lipopeptides (particle size: 300-500 nm) revealed that construct consisting higher rhamnose (trirhamnosyl-lipopeptide) was able to induce enhanced activity in mice, GlcNAc moiety not found be essential component GAC mimicked epitope. Trirhamnosyl-lipopeptide also showed 75-97% opsonic against four different clinical isolates GAS comparable peptide vaccine (J8-lipopeptide) which illustrated 65-96% activity.