A Single-Step Gold Nanoparticle–Blood Serum Interaction Assay Reveals Humoral Immunity Development and Immune Status of Animals from Neonates to Adults

• DOI: 10.1021/acsinfecdis.8b00213
• Publication Date: 2018-12-06T23:07:03Z
• Authors: Tianyu Zheng; John Crews; Jodi L. McGill; Kunal Dhume; Caroline Finn; Tara Strutt; Karl K. McKinstry; Qun Huo
• Keywords: Humoral immunity

A well-developed, functional immune system is paramount to combat harmful attacks from pathogenic organisms and prevent infectious diseases. Newborn animals humans have only limited immunity upon birth, but their functions are expected develop within weeks months eventually reach a maturity that will provide full protection. Despite the importance of activity in animal human health management, there no convenient test available allows for rapid assessment state function nonlaboratory settings. Here we report an extremely simple blood may be used point-of-care clinics or field settings evaluate humoral status animals. The detects cooperative interaction between gold nanoparticle arguably three most important proteins involved system: immunoglobulin M (IgM), G (IgG), at least one complement protein, C3, serum. Such interactions cause nanoparticles form clusters aggregates. average particle size nanoparticle–serum mixture, measured by dynamic light scattering, corresponds positively subject. Our study demonstrates not monitoring development neonates adults, also detecting active responses during infection. Although data reported here largely based on murine bovine models, it likely this applicable as well.