Confinement of Triple-Enzyme-Involved Antioxidant Cascade in Two-Dimensional Nanostructure

• DOI: 10.1021/acsmaterialslett.2c00580
• Publication Date: 2023-01-18T14:36:45Z
• Authors: Adel Szerlauth; Árpád Varga; Tamara Madácsy; Dániel Sebők; Sahra Bashiri; Mariusz Skwarczynski; Istvan Toth; József Maléth; Istvan Szilagyi
• Keywords: Horseradish peroxidase

Application of antioxidant enzymes in medical or industrial processes is limited due to their high sensitivity environmental conditions. Incorporation such nanostructures provides a promising route obtain highly efficient and robust biocatalytic system scavenge reactive oxygen species (ROS). Here, this question was addressed by confinement superoxide dismutase (SOD), horseradish peroxidase (HRP), catalase (CAT) into containing polyelectrolyte building blocks (alginate (Alg) trimethyl chitosan (TMC)) delaminated layered double hydroxide (dLDH) nanoparticle support. The nanocomposite possessed excellent structural colloidal stability, while tests revealed that the remained active upon immobilization developed composite greatly reduced intracellular oxidative stress two-dimensional cell cultures. Moreover, it effectively prevented hydrogen peroxide-induced stranded DNA breaks, which common consequence stress. results provide important tools design complex with multienzymatic activities for ROS scavenging.