Ribosome Fingerprinting with a Solid-State Nanopore

• DOI: 10.1021/acssensors.0c01642
• Publication Date: 2020-10-28T11:41:48Z
• Authors: Mukhil Raveendran; Anna Rose Leach; Tayah Hopes; Julie L. Aspden; Paolo Actis
• Keywords: Polysome; Nanopore; Eukaryotic Ribosome

Nanopores hold great potential for the analysis of complex biological molecules at single-entity level. One particularly interesting macromolecular machine is ribosome, responsible translating mRNAs into proteins. In this study, we use a solid-state nanopore to fingerprint 80S ribosomes and polysomes from human neuronal cell line andDrosophila melanogaster cultured cells ovaries. Specifically, show that peak amplitude dwell time characteristics are distinct can be used discriminate in mixed samples. Moreover, able distinguish large polysomes, containing more than seven ribosomes, those two three demonstrate correlation between polysome size amplitude. This study highlights application nanopores as rapid analytical tool detection characterization ribosomal complexes.