HologLev: A Hybrid Magnetic Levitation Platform Integrated with Lensless Holographic Microscopy for Density-Based Cell Analysis

• DOI: 10.1021/acssensors.0c02587
• Publication Date: 2021-06-14T14:40:51Z
• Authors: Kerem Delikoyun; Sena Yaman; Esra Yilmaz; Oyku Sarigil; Muge Anil-Inevi; Kubra Telli; Ozden Yalcin-Ozuysal; Engin Ozcivici; H. Cumhur Tekin
• Keywords: Magnetic Levitation

In clinical practice, a variety of diagnostic applications require the identification target cells. Density has been used as physical marker to distinguish cell populations since metabolic activities could alter densities. Magnetic levitation offers great promise for separating cells at single level within heterogeneous with respect Traditional magnetic platforms need bulky and precise optical microscopes visualize levitated Moreover, evaluation process densities is cumbersome, which also requires trained personnel operation. this work, we introduce device (HologLev) fusion principle lensless digital inline holographic microscopy (LDIHM). LDIHM provides ease use by getting rid expensive optics. By placing an imaging sensor just beneath microcapillary channel without any lenses, recorded holograms are processed determining through fully automated image processing scheme. The costs less than $100 compact design that can fit into pocket. We perform viability tests on levitating three different lines (MDA-MB-231, U937, D1 ORL UVA) comparing them against their dead correspondents. tested differentiation mouse osteoblastic (7F2) monitoring characteristic variations in density. Last, response MDA-MB-231 cancer chemotherapy drug was demonstrated our platform. HologLev cost-effective, label-free, analysis be highly desirable laboratory point-of-care testing applications.