Enhanced Microfluidic Sample Homogeneity and Improved Antibody-Based Assay Kinetics Due to Magnetic Mixing

• DOI: 10.1021/acssensors.1c00050
• Publication Date: 2021-06-30T19:49:06Z
• Authors: Eriola-Sophia Shanko; Lennard Ceelen; Ye Wang; Yoeri van de Burgt; Jaap den Toonder
• Keywords: Micromixer

Recent global events have distinctly demonstrated the need for fast diagnostic analysis of targets in a liquid sample. However, microfluidic lab-on-a-chip devices point-of-care diagnostics can suffer from slow due to poor mixing. Here, we experimentally explore mixing effect within chamber, as obtained superparamagnetic beads exposed an out-of-plane (vertical) rotating magnetic field. Various protocols are explored, and level sample homogeneity is measured by determining efficiency index. In particular, introduce method induce effective chamber actuation same perform swarming behavior, collective motion large number individual entities often seen nature. The microparticle induces high fluid velocities initially stagnant fluids, it be externally controlled. pilot-tested using test featuring bioluminescent assay detection antibodies. leads increased kinetics, which indeed reduces time sensor readout substantially. Magnetic expected beneficial wide variety devices, where reagents does play role.