Wide-Range, Rapid, and Specific Identification of Pathogenic Bacteria by Surface-Enhanced Raman Spectroscopy

• DOI: 10.1021/acssensors.1c00641
• Publication Date: 2021-07-20T15:39:42Z
• Authors: Siying Liu; Qiushi Hu; Chao Li; Fangrong Zhang; Hongjing Gu; Xinrui Wang; Shuang Li; Lei Xue; Tobias Madl; Yun Zhang; Lei Zhou
• Keywords: Pathogenic bacteria; Surface-Enhanced Raman Spectroscopy

Sensitive, selective, rapid, and label-free detection of pathogenic bacteria with high generality is great importance for clinical diagnosis, biosecurity, public health. However, most traditional approaches, such as microbial cultures, are time-consuming laborious. To circumvent these problems, surface-enhanced Raman spectroscopy (SERS) appears to be a powerful technique characterize at the single-cell level. Here, by SERS, we report strategy rapid specific 22 strains common bacteria. A novel high-quality silver nanorod SERS substrate, prepared facile interface self-assembly method, was utilized acquire chemical fingerprint information pathogens improved sensitivity. We also applied mathematical analysis methods, t-test receiver operating characteristic determine features demonstrate clear identification (20 strains) from rest reliability this sensor. This wide-range bacterial offers significant advantages over existing approaches sets base automated onsite in complex real-life situation.