A Multiplexable Plasmonic Hairpin-DNA Sensor Based On Target-specific Tether Dynamics

• DOI: 10.1021/acssensors.1c02097
• Publication Date: 2021-12-01T18:59:56Z
• Authors: Jeanne Elisabeth ...; Laurens Rudi Spoe...; Johanna Theodora ...; Joshua Taylor Loe...; Jan Cornelis Titu...; Loes Irene Segerink
• Keywords: Multiplex

The need for measurements of multiple biomarkers simultaneously at subnanomolar concentrations asks the development new sensors with high sensitivity, specificity, precision, and accuracy. Currently, multiplexed sensing in single molecule increases complexity system terms reagents sample read-out. In this letter, we propose a novel approach to multiplex hairpin-based single-DNA sensors, which overcomes limitations present approaches multiplexing. By target-dependent ssDNA hairpin design, can create DNA tethers that have distinct tether dynamics upon target binding. Our numerical model shows by changing stem length hairpin, significantly different dynamic behavior will be observed. exploiting distance-dependent coupling AuNPs gold films, probe along z-axis using simple laser equipped microscope.