A Non-Immunized and BSA-Template Aggregation-Induced Emission Sensor for Noninvasive Detection of Cystatin C in the Clinical Diagnosis of Diabetes Nephropathy

• DOI: 10.1021/acssensors.2c02122
• Publication Date: 2023-03-06T16:21:00Z
• Authors: Shujing Chen; Kunze Du; Shuangqi Wang; Chunxiao Liang; Ye Shang; Xiaoyuan Xie; Ge Tang; Jin Li; Bing Wang; Xie-an Yu; Yanxu Chang
• Keywords: Bovine serum albumin

Diabetes nephropathy (DN) is one of the main causes death in patients with diabetes. Cystatin C (Cys C) a reliable indicator glomerular filtration function. Therefore, it urgent and meaningful to obtain early warning DN by noninvasive measurement Cys C. In this investigation, novel fluorescence sensor (BSA-AIEgen sensor) was synthesized cross-linking aggregation-induced emission (AIE) characteristics 2-(4-bromophenyl)-3-(4-(4-(diphenylamino) styryl) phenyl) fumaronitrile (TPABDFN) bovine serum albumin (BSA), which exhibited "On" state owing restriction intramolecular motions (RIM) phenomenon TPABDFN. Intriguingly, decrease BSA-AIEgen sensors could be found BSA on surface hydrolyzed papain, but reverse emerged increase content as inhibitor papain. Hence, successfully detected employing fluorescent differential display linear range from 12.5 ng/mL 800 (R2 = 0.994) limit detection (LOD) 7.10 (S/N 3). Further, developed differentiates diabetes volunteers advantages high specificity, low cost, simple operation. Accordingly, expected become non-immunized method monitor for warning, diagnosis, drug efficacy evaluation nephropathy.