Continuous Ketone Monitoring via Wearable Microneedle Patch Platform

• DOI: 10.1021/acssensors.3c02677
• Publication Date: 2024-02-01T17:59:43Z
• Authors: Chochanon Moonla; Maria Reynoso; Ana Casanova; An-Yi Chang; Omeed Djassemi; Aishwarya Balaje; Amal Abbas; Zhengxing Li; Kuldeep Mahato; Joseph Wang
• Keywords: Wearable Electronic Devices; 3-Hydroxybutyric Acid; Nanotubes, Carbon; Humans; Pilot Projects; Ketone Bodies; Ketones

Ketone bodies (KBs), especially β-hydroxybutyrate (BHB), have gained tremendous attention as potential biomarkers their presence in bodily fluids is closely associated with health and wellness. While a variety of blood fingerstick test strips are available for self-testing BHB, there major needs wearable devices capable continuously tracking changing BHB concentrations. To address these needs, we present here the first demonstration microneedle-based continuous ketone monitoring (CKM) human interstitial fluid (ISF) illustrate its ability to follow intake drinks. ensure highly stable selective detection ISF new enzymatic microneedle sensor relies on gold-coated platinum working electrode modified reagent layer containing toluidine blue O (TBO) redox mediator, dehydrogenase (HBD) enzyme, nicotinamide adenine dinucleotide (NAD+) cofactor, along carbon nanotubes (CNTs), chitosan (Chit), poly(vinyl chloride) (PVC) outer protective layer. The skin-worn sensing device operates miniaturized electrochemical analyzer connected wirelessly mobile electronic capturing, processing, displaying data. Cytotoxicity skin penetration studies indicate absence harmful effects. A pilot study involving multiple subjects evaluated ISF, against gold standard meter measurements, revealing close correlation between two methods. Such CKM offers considerable promise dynamic toward management diabetic ketoacidosis personal nutrition