Multiplexing Label-Free Polymeric Nanocarriers via Antipolymer Antibodies

• DOI: 10.1021/acssensors.4c03184
• Publication Date: 2025-01-31T03:33:16Z
• Authors: James Humphries; Jody Hobson-Peters; Saikat Ghosh; Christopher B. Ho...; Pie Huda; Craig A. Bell; Nicholas L. Fletcher; Kristian Kempe; Kristofer J. Thur...
• Keywords: Nanocarriers; Antibody therapy

Recent examples of immune responses directed against the synthetic polymer poly(ethylene glycol) (PEG) have led to development biocompatible polymers, which are viewed as promising candidates act surrogate materials for use in biological applications, such hydrophilic poly(2-oxazoline)s (POx). Despite this, characterization critical aspects response these emerging is sparse, part because no known monoclonal antibodies (mAbs) this family material been reported. To advance understanding responses, we report successful isolation and hybridoma-derived mAbs with excellent specificity different POx species notable selectivity highly branched architectures over linear systems. In conjunction established targeted PEG, show that can be employed sensitive vivo multiplex-detection label-free therapeutics based on polymer-antibody binding. This approach enables scalable therapeutic drug monitoring multiple within a single animal, simultaneously.