Detection of K+ Efflux from Stimulated Cortical Neurons by an Aptamer-Modified Silicon Nanowire Field-Effect Transistor

• DOI: 10.1021/acssensors.6b00505
• Publication Date: 2016-12-24T12:21:22Z
• Authors: Ankur Anand; Chia-Rung Liu; Ai-Chuan Chou; Wan-Hsuan Hsu; Rajesh Kumar Ulag...; Yi-Cheng Lin; Chi-An Dai; Fan-Gang Tseng; Chien-Yuan Pan; Yit-Tsong Chen
• Keywords: Aptamer

The concentration gradient of K+ across the cell membrane a neuron determines its resting potential and excitability. During neurotransmission, efflux from via various channels will not only decrease intracellular content but also elevate extracellular concentration. However, it is clear to what extent this change could be. In study, we developed multiple-parallel-connected silicon nanowire field-effect transistor (SiNW-FET) modified with K+-specific DNA-aptamers (aptamer/SiNW-FET) for real-time detection cultured cortical neurons. aptamer/SiNW-FET showed an association constant (2.18 ± 0.44) × 106 M–1 against either less or negligible response other alkali metal ions. α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) stimulation induced outward current hyperpolarized in whole-cell patched under Na+/K+-free buffer. When neurons were placed atop buffer, AMPA (13 μM) elevated ∼800 nM, which greatly reduced by 6,7-dinitroquinoxaline-2,3-dione, receptor antagonist. EC50 elevating was 10.3 μM. By stimulating normal physiological isolated cytosolic fraction decreased 75%. These experiments demonstrate that sensitive detecting cations concentrations inside outside be changed modulate